Journal: Advanced Materials (Deerfield Beach, Fla.)
Article Title: Deployable 3D‐Printed Vascular Stent with Surface‐Catalysed Endogenous Nitric Oxide Generation
doi: 10.1002/adma.202520199
Figure Lengend Snippet: In vitro biocompatibility and intracellular NO generation characterisation of DSENO materials. (a, b) Cell viability of HUVECs (a) and HCASMCs (b) after 48 h incubation with printed DSENO plates. (c) Quantitative analysis of material‐induced intracellular NO generation. Endogenous NO production in HUVECs was quantified by measuring the mean fluorescence intensity (MFI) of the DAF‐FM probe using high‐resolution confocal laser scanning microscopy (CLSM). (d) CLSM images of HUVECs after 48 h incubation with material candidates, cells stained with DAF‐FM (green) and Hoechst (blue) ( n ≥ 6). Scale bar = 100 µm. Data are normalized to the blank control and represent the average MFI of multiple regions of interest. Error bars indicate standard deviation; statistical significance was determined via one‐way ANOVA ( * p < 0.05).
Article Snippet: Human coronary artery smooth muscle cells (HCASMCs) from Cell Applications were maintained in human smooth muscle cell growth medium (311–500) and passaged at 70–90% confluency.
Techniques: In Vitro, Incubation, Fluorescence, Confocal Laser Scanning Microscopy, Staining, Control, Standard Deviation